By putting the pointed end of the pipette just over an Amœba, for example, and sucking gently at the other end, we shall draw some water and the animal into the swollen part of the tube.
If we then place one finger over the end of the pipette to which we have applied suction we can transfer the contents wherever we wish without it running out, as soon as we remove our finger the pipette empties itself.
Hold the pipette between the thumb and second finger of the right hand with the tip below the surface of the milk, draw the milk by suction with the lips until it is filled well above the graduation.
Roqueforti spores in water into a sterile pipette (10 c.
The acid test is conducted as with milk (see Chapter II) except the starter must be rinsed out of the pipette with pure water.
The sample to be tested is measured with the pipette the same as the milk and placed in the skimmed-milk test bottle.
To obtain an accurate reading, the pipette should be on a level with the eye.
Then with the left hand, hold the milk test bottle in a slanting position and place the tip of the pipette into it about one-third of an inch and at a slight angle.
A pipette or slender-spouted vessel may be used to add the water.
With the aid of a pipettecover these spots with water and let it remain for ten or fifteen minutes; then with the pipette remove the liquid and examine the products it holds in solution.
Herz, in which the clotting of the blood in the pipette is prevented by rendering the walls absolutely smooth by the application of cod-liver oil.
Of the instruments for measuring the depth of colour of the blood, the double pipette of Hoppe-Seyler is quite the most delicate.
It consists of a small retort or evaporating vessel made out of a pipette of 200 c.
The pipette is next held over the vessel into which the 100 c.
The flow of liquid from a pipette must not be hastened by blowing into it.
Warm until any precipitate which has formed is redissolved; allow to cool slowly, and run in from a pipette a weak solution of permanganate until a faint brown colour is produced.
The other form is the one in general use; in fact, a long nose to a pipette is so convenient that it may almost be said to be necessary.
Fill the pipette with dilute acid, close the clamp, and cork the flask.
This is most conveniently filled by stopping the jet with the finger, and allowing the liquid to flow in a fine stream into the neck until the pipette is filled, and then working as just described.
All measurements should be made at the ordinary temperature; and, before being used, the pipette should be rinsed out with a cubic centimetre or so of the solution to be measured.
On this account the standard solution is run in from a special pipette capable of delivering a practically invariable volume of solution.
Run in through the pipette (by opening the clip) 20 c.
The neck of the pipette should be long enough to allow its being firmly grasped by the fingers and thumb of the right hand without inconvenience.
The small flask is fitted with a rubber-stopper perforated with two holes, through one of which passes the tube of a pipette holding 25 or 30 c.
The pipette is then withdrawn; it is in the withdrawing that the disadvantage of this particular form[5] makes itself felt.
As soon as the solution forms a supernatant layer upon the surface of the fluid, it is drawn off with a pipette and evaporated on a water-bath.
The filtrate is next supersaturated with ammonia, and well shaken with chloroform, which, upon being separated by means of a pipette and evaporated, leaves the alkaloid in an impure state.
The two branches of the gauge, as well as the pipette itself, are graduated into equal divisions.
So the Oven Door had to remain an unsolved mystery, and Pip and Pipette were compelled to comfort themselves with the Talking-Hole.
This was the last occasion in their lives on which Pipette ever questioned the wisdom of her beloved brother's actions.
After that Pipette became engaged, and the curtain fell upon Elsie's movements, for Pipette's letters now harped upon a single string, and Pip was far too shy to ask for information outright.
Apparently being "sensible" did not include abstinence from tears, forPipette was now weeping softly.
After that Pip would be the patient, Pipette Mr. Evans, and Tattie Father, and the performance was repeated in extenso.
Presently Pipette returned, carrying two little basins of the soup which usually served to span the yawning gulf between their breakfast and dinner.
Presently Pipette slipped a soft and protecting arm round her father's neck.
This time, in turning the call-handle, Pipette accidentally placed her hand on the receiver-hook, with the result that she actually rang up the Exchange Office.
The solution in each pipetteshould be drawn up to the mark on the capillary tube.
The gas is then driven over into the pipette C and a similar operation is carried out.
Each pipette contains a number of glass tubes, to which some of the solution clings, thus facilitating the absorption of the gas.
The rear half of each pipette is fitted with a rubber bag, one of which is shown at K, to protect the solution from the action of the air.
The cock G is then closed and the cock I in the pipette B is opened and the gas driven over into B by raising the bottle F.
Then with a pipette we introduce into the solution, in a regular pattern, a number of drops of liquid coloured with Indian ink.
A Grethan or Lunge pipette will be found convenient.
Conduct 2 or 3 blank determinations, using the same pipette and draining for the same length of time as above.
Allow the lyes to settle and with an inverted pipette draw off the lyes into a test tube or bottle.
The capillary hydrometer consists simply of a small pipette with a bulb in the middle of the stem, the pipette terminating in a very fine capillary point.
If danger of more carbonic acid being absorbed from the air is feared, and always in very accurate analysis, a potassa tube may be connected to the pipette before drawing the air through.
When fairly cold the liquid may be withdrawn by a pipette attached to the tube.
Allow the water to drain off and add Gram’s iodine slowly from a pipette so as not to disarrange the section.
If the upper benzene layer is fully gelatinous and emulsive, the under layer of water is to be removed with a pipette as far as possible, and the benzene with a few drops of absolute alcohol and filtration.
Put one end of the pipette into the milk sample and the other end into the mouth.
As soon as the mark is reached, withdraw the pipette from the mouth and quickly press the forefinger on the mouth end.
Suck milk into the pipette until the milk comes up to the mark on the side of the pipette.
Then put the lower end of the pipette into one of the small long-necked bottles of the machine, and, lifting the finger, allow the milk to flow gently into the bottle.
Acidity of Lemons With a pipette measure into a small beaker 2 cc.
The shank also passes through a stuffing-box of cotton-wool moistened with glycerine; so that, tightly clasped by the rubber and wool, the pipette is not likely in its motions up and down to carry any dust into the chamber.
To most patients the application with the pipette may be too difficult or impossible; I have therefore already suggested the use of Weber's very simple but effective nose-douche.
I then lay flat on my back, keeping my head very low, and poured with a pipette about four cubic centimetres into both nostrils.
When the fluid in the pipette has fallen to the required level, the drops which may happen to adhere to the outside of the pipette are carefully wiped off, and the contents of the tube are then fully transferred to the other vessel.
Thus constructed, the lower end of the pipette can be dipped into a vessel with a narrow and long neck, such as a flask, containing a fluid, the required volume of which can be removed from it.
The pipette varies in capacity from 1 to 200 cubic centimetres.
The pipette mostly consists of a bulb, from each end of which proceeds a straight, slender hollow stem, communicating with the bulb, and varying in length with the capacity of the instrument.
In this new process the pipette is filled from the top like a bottle, instead of being filled by suction, and it is moreover fixed.
We next apply neatly the forefinger of one hand to this orifice, remove the pipette from the liquid, and seize it as represented in fig.
We say purposely at one efflux, because after the cessation of the jet, the pipette may still furnish two or three drops of liquid, which must not be counted or reckoned upon.
Illustration: 83 84] After having immersed the beak c of the pipette in the solution, we apply suction by the mouth, to the upper orifice, and thereby raise the liquid to d above the circular line a b.
Collect a sample of pericardial fluid in a pipette for microscopical and cultural examination.
If using a pipette with a constriction below the plugged mouthpiece (Fig 13b), this portion of the pipette may also be sealed in the flame.
Remove the tubes from the incubator and into each tube pipette 0.
Centrifugalise again, pipette off the supernatant fluid; fill the tube with sterile water; distribute the deposit evenly throughout the fluid, and transfer the suspension to a litre flask.
Slightly raise the cover of the dish, and with a sterile teat-pipette deposit a small drop of sterile water in the centre of each of the nine divisions.
Pipette off the clear serum, and fill in quantities of 1 c.
Bent syphon tube, with pipette nozzle attached by means of rubber tubing and fitted with pinch-cock.
Aspirate into the pipette one volume of washed cells, air index, one volume of bacterial emulsion, air index, and one volume of specific serum (see Fig.
Withdraw thepipette from the bottle, replace the stopper, and stand the bottle upright.
Maintaining the pressure on the teat pass the stem of the pipette into the capsule holding the saline solution, until the open end of the pipette is below the level of the fluid.
Remove the point of the pipette from the secretion, allow the fluid to run a short distance up the capillary stem and seal the point of the pipette in the flame.
By marking the blood pipette above the level of the serum with the glass cutting knife and snapping the tube at that point, the blood-serum becomes readily accessible for testing purposes.
When ready to examine the morbid material snap off the sealed end of the pipette with sterile forceps and eject the contents of the pipette into a sterile capsule.
Now pass the point of the pipette into the blood tube and dip the open end below the surface of the serum.
Then run in the permanganate solution from a pipette or burette to determine roughly the amount required.
Hold the water at that point by pressure of the finger and then allow the water to run out from the pipette into a small tared, or weighed, beaker or flask.
Empty the flask or pipette into a beaker and wash it out.
After a definite time interval, usually two to three minutes, touch the end of the pipette against the side of the beaker or flask to remove any liquid adhering to it (Note 1).
The form of the pipette varies with the nature of the absorbing material.
There it is allowed to remain, the pipette being shaken from time to time.
For solutions which remain permanent in air the two-bulbed form suffices; in other cases a composite pipette (fig.
Any number of bulbs can be attached to the horizontal capillary; in the form illustrated there are four, the last being a hydrogen pipette in which the palladium is heated in a horizontal tube by a spirit lamp.
Oxygen is absorbed by stick phosphorus contained in a tubulated pipette filled with water.
After standing for twenty-four hours a little of the sediment is taken by means of a pipette and examined by ordinary methods, though after "fixing" the films with heat they are some times passed through equal parts of alcohol and ether.
This consists in drawing up into a fine sterilised pipette a small quantity of the water and introducing it thereby into a test-tube of melted gelatine at a temperature below 40° C.
