The Trios are not mentioned in the first letter, in which Beethoven offers the Fifth and Sixth Symphonies besides other works to Breitkopf and Haertel.
And, to mention in passing a very ingenious experiment of Pasteur's, we thus obtain a means of separating by culture the bacillus of splenic fever from the septic vibrio when they are temporarily associated together.
The others diffuse themselves more or less slowly in the blood, but the septic vibrio takes almost immediate possession of the dead body.
In contact with air the cultivations of septic vibrios would prove sterile, because the vibrio is exclusively anaerobic and air kills it.
In the vibrio of acute septicæmia this is the mode of generation.
And this septicæmia is sometimes manifold, for a special septicæmia may be said to correspond to every sort of vibrio of putrefaction.
There the filamentous vibrio dies, and disappears under the form of fine amorphous granulations deprived of virulence.
If this same mixture is cultivated without air, either in a vacuum or in carbonic acid gas, the septic vibrio alone will be developed.
These cultivations, however, of the septic vibrio require very special precautions and conditions.
A worm known by the name of Vibrio anguillula lives in grains of corn while still green, and multiplies there to a prodigious extent; it is this which causes the disease known by the name of smut.
To this end, we attempted the cultivation of the septic vibrio from an animal dead of septicemia.
It is impossible, then, to assert that there is a separate virulent substance, either fluid or solid, existing, apart from the adult vibrio or its germ.
It occurred to us that the septic vibrio might be an obligatory anaerobe and that the sterility of our inoculated culture fluids might be due to the destruction of the septic vibrio by the atmospheric oxygen dissolved in the fluids.
Results justified our attempt; the septic vibrio grew easily in a complete vacuum, and no less easily in the presence of pure carbonic acid.
The Academy may remember that I have previously demonstrated facts of this nature in regard to the vibrio of butyric fermentation, which not only lives without air but is killed by the air.
The lochia were full of the pyogenic vibrio and of the organism of furuncles, although there was but a small proportion of the latter.
The vibrio is destroyed by oxygen, and it is only where it is in a thick layer that it is transformed to germ-corpuscles in the presence of oxygen and that its virulence is preserved.
It was necessary therefore to attempt to cultivate the septic vibrio either in a vacuum or in the presence of inert gases--such as carbonic acid.
Our researches concerning the septic vibrio had not so far been convincing, and it was to fill up this gap that we resumed our experiments.
When the vibrio encounters an obstacle it turns, or after assuring itself by some visual effort or other that it cannot overcome it, it retraces its steps.
For some years it was called the "comma bacillus," from its supposed resemblance in shape to a comma, but it was subsequently found to be a vibrio or spirillum, not a bacillus.
The discovery was received with much scepticism in some quarters, and the claim of Koch's vibrio to be the true cause of cholera was long disputed, but is now universally acknowledged.
In the meanwhile (1892) cholera had made its appearance in France; the specificity of the cholera vibriowas not finally established at that time.
The preceding results having led him to suppose that the cholera vibrio became attenuated in vitro and might perhaps serve as a vaccine against cholera, he gave a culture of long standing to the young volunteer.
As he failed to solve the problem of the specificity of the cholera vibrio on animals, he resolved to experiment upon himself and consumed a culture of cholera vibriones.
Similar experiments within the organism of animals gave no conclusive results; the simultaneous ingestion of the cholera vibrio and of favourable microbes did not induce cholera.
He sowed the cholera vibrio with divers other microbes and saw that some of them facilitated its culture whilst others prevented it.
It is very ingenious," said I, really appreciating the difficulty of being lively in this connection: it seemed even more wonderful than that a Vibrio should have an eye.
Vibrio is a poor little tippling creature, but, as Mr Carlyle would say, he has an eye, and he is always lively.
The same physiologist discovered animalcules in the pus of certain ulcers not dissimilar in appearance to the vibrio lineola of Mueller.
Snails have been thus reanimated after a lapse of fifteen years; and Bauer revived the Vibrio tritici, after an apparent death of five years and eight months, by merely soaking it in water.
