It may be heated in theautoclave for ten to fifteen minutes at 10 pounds' pressure and sometimes not be hydrolyzed, but the procedure is uncertain and very resistant spores may not be killed.
A copper water bath with a tightly closed air compartment or the horizontal autoclave (Fig.
The agar medium may be sterilized in the autoclave for fifteen minutes at 15 pounds pressure as the high temperature does not injure the agar.
The solidified serum in either case is best sterilized discontinuously, though with care the autoclave at 15 pounds pressure may be used for a single sterilization.
Heat the whole apparatus in the autoclave at 120° C.
Fix the bucket over a large Bunsen flame and boil for thirty minutes--or boil in the autoclave for a similar period.
Pack the tubes in the wire basket of the autoclave (having previously removed the cotton-wool plugs, caps, etc.
Sterilisation by means of superheated steam is carried out in a special boiler--Chamberland's autoclave (Fig.
See that the rubber washer is in position, then replace the cover and fasten it tightly on to the autoclave by means of the thumbscrews.
If an autoclave is not available, the tubes must be placed in a digester, or even a large pan or pail with a tightly fitting cover, and boiled vigorously for some thirty to forty-five minutes to ensure disinfection.
While the introduction of the Twitchell process to a great extent replaced the autoclave method of saponification for obtaining fatty acids for soap making, the autoclave method is also used.
When the saponification is completed the contents of the autoclave are removed, usually by blowing out the digestor into a wooden settling tank, or by first running off the glycerine water and then blowing out the lime, soap and fatty acids.
This is a much higher pressure than when lime is used and therefore a very strong autoclave is required.
To charge the autoclave a partial vacuum is created therein by condensation of steam just before running the purified oil in from an elevated tank.
Z Zinc oxide, Use of inautoclave saponification, 120.
The method consists in merely charging the autoclave with fats and adding about 30 per cent.
The saponification in an autoclave is usually carried out by introducing the fats into the autoclave with a percentage of lime, magnesia or zinc oxide, together with water.
The advantage of autoclave saponification over the Twitchell process is that a greater cleavage of the fats and oils results in less time and at a slightly less expense.
An autoclave or digestor consists of a strongly constructed, closed cylindrical tank, usually made of copper, and is so built as to resist internal pressure.
It has also been proposed to use zinc oxide in conjunction with lime in the autoclave to obviate to some extent the discoloration of the fatty acids.
French saponification or "candle crude" glycerine is the result of concentration of "sweet water" produced in the manufacture of stearine and by the autoclave process.
The above list will hopefully give you a few useful examples demonstrating the appropriate usage of "autoclave" in a variety of sentences. We hope that you will now be able to make sentences using this word. Other words: boil; broom; cleaning; device; dish; fumigate; receptacle; sterilize
